物理化学学报 >> 2003, Vol. 19 >> Issue (09): 800-804.doi: 10.3866/PKU.WHXB20030904

研究论文 上一篇    下一篇

嗜盐古菌染色体DNA片段在大肠杆菌中的启动子功能

刘义;黄玉屏;高振霆;段珍红;沈萍;屈松生   

  1. 武汉大学 1化学与分子科学学院化学系, 2生命科学学院, 武汉　430072
  • 收稿日期:2002-12-06 修回日期:2003-04-01 发布日期:2003-09-15
  • 通讯作者: 刘义 E-mail:liuyi@public.wh.hb.cn

Promoter Function of Halobacterium halobium Chromosome DNA Fragment in Escherichia coli

Liu Yi;Huang Yu-Ping;Gao Zhen-Ting;Duan Zhen-Hong;Shen Ping;Qu Song-Sheng   

  1. Department of Chemistry, College of Chemistry and Molecular Sciences, 2College of Life Sciences, Wuhan University, Wuhan　430072
  • Received:2002-12-06 Revised:2003-04-01 Published:2003-09-15
  • Contact: Liu Yi E-mail:liuyi@public.wh.hb.cn

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摘要： 以启动子探针质粒pKK232-8为载体, 用微量量热法研究了源自盐生盐杆菌R1染色体的RM13 DNA片段在大肠杆菌HB101中的真细菌启动子功能, 该启动子RM13 DNA片段的大小为1000bp(碱基对), 它能启动探针质粒pKK232-8上的氯霉素乙酰水解酶基因(即:氯霉素抗性基因Cmr), 氯霉素抗性水平可达到150 mg•L－1, 抗性水平较高, 启动活性较大.研究结果表明, 在盐生盐杆菌染色体上可能存在具有双功能或多功能的启动子DNA片段, 这对系统发育、微生物遗传学和生物热化学等均有重要的意义.

关键词: 盐生盐杆菌R1, 大肠杆菌HB101, 质粒pKK232-8, 启动子功能, 微量量热

Abstract: A promoter-probe plasmid pKK232-8 was used as a vector, which functioned in eubacteria Escherichia(E.) coli HB101. The promoter function of the chromosome DNA fragment from Halobacterium(H.) halobium R1, RM13 DNA fragment, active as eubacteria promoters in E.coli HB101, was studied by microcalorimetry. The promoter is of about 1000 bp(base pair).It can promote the chloramphenicol acetyltransferase (CAT) gene in plasmid pKK232-8, and the chloramphenicol resistance level is about 150 mg•L－1.The result indicates that there are probably double-function promoters in H.halobium chromosome.

Key words: Halobacterium halobium R1, Escherichia coli HB101, Plasmid pKK232-8, Eubacterial promoter function, Microcalorimetry