物理化学学报 >> 2008, Vol. 24 >> Issue (06): 1068-1072.doi: 10.3866/PKU.WHXB20080627

研究论文 上一篇    下一篇

TATP-铜(II)-L-丝氨酸(L-精氨酸)配合物与DNA的相互作用

古琴; 任祥祥; 乐学义   

  1. 华南农业大学理学院应用化学系, 广州 510642
  • 收稿日期:2007-12-24 修回日期:2008-02-27 发布日期:2008-06-03
  • 通讯作者: 乐学义 E-mail:lexy@scau.edu.cn

Interaction of 1,4,8,9-Tetraazatriphenylene-Copper (II)-L-Serinate (L-Argininate) Complexes with DNA

GU Qin; REN Xiang-Xiang; LE Xue-Yi   

  1. Department of Applied Chemistry, College of Sciences, South China Agricultural University, Guangzhou 510642, P. R. China
  • Received:2007-12-24 Revised:2008-02-27 Published:2008-06-03
  • Contact: LE Xue-Yi E-mail:lexy@scau.edu.cn

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摘要： 采用电子吸收光谱、荧光光谱、粘度测定和琼脂糖凝胶电泳方法研究了配合物[Cu(TATP)(L-Ser)(H2O)]·ClO4(1)和[Cu(TATP)(L-Arg)(H2O)]2ClO4·0.5H2O(2)(TATP=1,4,8,9-四氮三联苯, L-Ser=L-丝氨酸, L-Arg=L-精氨酸)与DNA之间的相互作用. 结果表明, 配合物电子吸收光谱的最大吸收峰在加入DNA后产生明显的减色效应, 配合物能极大地淬灭溴化乙啶(EB)-DNA体系的荧光, DNA的粘度随配合物浓度的增加而增大, 表明配合物对DNA有较强的插入作用, 作用力大小为配合物2>1; 另外, 凝胶电泳实验结果表明配合物在维生素C存在的条件下对pBR322 DNA具有显著的断裂作用.

关键词: 铜(II)三元配合物, 1,4,8,9-四氮三联苯, L-α-氨基酸, DNA

Abstract: Interactions of ternary Cu(II) complexes [Cu(TATP)(L-Ser)(H2O)]·ClO4(1) and [Cu(TATP)(L-Arg) (H2O)]2ClO4·0.5H2O (2) (TATP=1,4,8,9-tetraazatriphenylene, L-Ser=L-serinate, L-Arg=L-argininate) with DNA have been investigated by electronic absorption spectroscopy, fluorescence spectroscopy, viscosity, and gel electrophoresis measurements. The intensity of maximal absorption peaks of absorption spectra of the complexes was weakened with the increase in DNA concentration, and the complexes could remarkably quench the emission intensity of ethidium bromide (EB)-DNA system. At the same time, the viscosity of DNA decreased with the increase in the concentration of the complexes. The results indicated that the intercalative interaction of the complexes with DNA was strong and gave the order of the binding abilities of the complexes to DNA: complex 2>1. Moreover, the result of agarose gel electrophoresis showed that the complexes had high cleaving efficiency to pBR322 DNA in the presence of vitamin C.

Key words: Ternary copper (II) complex, 1,4,8,9-tetraazatriphenylene, L-α-amino acid, DNA