物理化学学报 >> 2010, Vol. 26 >> Issue (05): 1442-1447.doi: 10.3866/PKU.WHXB20100525

生物物理化学 上一篇    下一篇

微量热法研究鹿茸4个活性部位对肠道特征菌群生长代谢的影响

唐慧英, 鄢丹, 张清哲, 张少锋, 肖小河, 刘荣华   

  1. 解放军中药研究所, 解放军第三O二医院, 北京 100039; 江西中医学院现代中药制剂教育部重点实验室, 南昌 330004; 中国中医科学院中药研究所, 北京 100700
  • 收稿日期:2009-10-22 修回日期:2010-01-17 发布日期:2010-04-29
  • 通讯作者: 鄢丹, 肖小河 E-mail:yd277@126.com

Microcalorimetric Investigation of Four Bioactive Extracts from Cornu Cervi Pantotrichumon Intestinal Diagnostic Flora Growth

TANG Hui-Ying, YAN Dan, ZHANG Qing-Zhe, ZHANG Shao-Feng, XIAO Xiao-He, LIU Rong-Hua   

  1. PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, P. R. China; Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, P. R. China; Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences, Beijing 100700, P. R. China
  • Received:2009-10-22 Revised:2010-01-17 Published:2010-04-29
  • Contact: YAN Dan, XIAO Xiao-He E-mail:yd277@126.com

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摘要：

建立灵敏表征鹿茸中具有补益活性的4个部位对两种肠道特征菌群生长代谢影响的评价方法. 采用微量热法, 在不同给药条件作用下, 以产热功率-时间曲线(热谱曲线)的特征参数(生长速率常数(k)、最大产热功率(Pmax)、达峰时间(tp)及有效率(E)等)为指标, 对肠道主要有益菌(青春双歧杆菌)生长代谢程度进行客观地量化评价. 挑选鹿茸促菌最强的活性部位, 进一步考察该部位对病原微生物(金黄色葡萄球菌)生长抑制的作用规律. 结果表明, 鹿茸不同活性部位促进青春双歧杆菌生长代谢强弱顺序为: 正丁醇>乙醚>氯仿>乙酸乙酯. 活性最强的正丁醇萃取液对病原微生物(金黄色葡萄球菌)抑制影响的研究表明, 正丁醇萃取液在浓度大于200 μg·mL-1时, 对金葡菌生长具有抑制作用. 且tp随药液浓度的增加而相应地延长; k, Pmax均随浓度的增加而相应地减小. 微量热法具有灵敏准确、实时在线、重现性好等特点, 可用于名贵药材——鹿茸的活性部位的筛选, 也为药效物质的研究提供了一个新的研究思路和方法.

关键词: 微量热法, 鹿茸, 青春双歧杆菌, 金黄色葡萄球菌, 肠道特征菌群, 活性部位

Abstract:

The facilitative effects of four bioactive extracts from Cornu Cervi Pantotrichum, a valuable traditional Chinese medicinal herb, on the growth of two kinds of intestinal diagnostic flora were investigated by the microcalorimetry. By analyzing the power-time curves, quantitative parameters, such as the growth rate constant (k), maximumheat output power (Pmax), peak time of maximumheat output power (tp), and effective power (E), were obtained to characterize the interactions of the extracts and Bifidobacterium adolescentis, which benefits the host animal by improving its intestinal microbial balance. The potential pharmacological action of the four bioactive extracts was analyzed to determine the strongest facilitative extract from Cornu Cervi Pantotrichum. The sequence of facilitative activity for the different extracts was: n-butanol > diethyl ether > chloroform > ethyl acetate (EtAc). The inhibitory effects of n-butanol extraction on the growth of Staphylococcus aureus, a harmful bacterium, were investigated. Results indicated that the concentration (200-1200 μg·mL-1) of n-butanol extraction notably affected the growth of this fungus. Furthermore, the value of tp increased as the concentration of n-butanol used in the extraction increased. However, k and Pmax decreased as the concentrations of the drugs increased. The results show that microcalorimetry is sensitive, accurate, rapid and convenient. This method can also be applied in real time and online to monitor the process of bacterial growth and can be used to screen the bioactive extracts of Cornu Cervi Pantotrichum. This method is also useful in providing some suggestions for the study of pharmacodynamic action.

Key words: Microcalorimetry, Cornu Cervi Pantotrichum, Bifidobacterium adolescentis, Staphylococcus aureus, Intestinal diagnostic flora, Bioactive part