超声作用下稀土离子对纳米TiO2与牛血清白蛋白相互作用的影响

doi:10.3866/PKU.WHXB201112051

物理化学学报 >> 2012, Vol. 28 >> Issue (02): 470-478.doi: 10.3866/PKU.WHXB201112051

超声作用下稀土离子对纳米TiO₂与牛血清白蛋白相互作用的影响

高晓亚, 文雯, 宋志英, 张爱平, 郝娟, 黄茜

山西医科大学药学院, 太原 030001

收稿日期:2011-08-24 修回日期:2011-11-25 发布日期:2012-01-11
通讯作者: 张爱平 E-mail:zhangap1@163.com
基金资助:
山西省自然科学基金(2010011048-1)和山西医科大学科技创新基金(01200806)资助项目

Effects of Rare Earth Ions on the Interaction between Nano TiO₂ and Bovine Serum Albumin in the Presence of Ultrasound

GAO Xiao-Ya, WEN Wen, SONG Zhi-Ying, ZHANG Ai-Ping, HAO Juan, HUANG Qian

Department of Pharmacy, Shanxi Medical University, Taiyuan 030001, P. R. China

Received:2011-08-24 Revised:2011-11-25 Published:2012-01-11
Contact: ZHANG Ai-Ping E-mail:zhangap1@163.com
Supported by:
The project was supported by the Natural Science Foundation of Shanxi Province, China (2010011048-1) and Technology Innovation Foundation of Shanxi Medical University, China (01200806).

摘要/Abstract

摘要： 在模拟人体生理条件下, 采用紫外光谱法、荧光光谱法和聚丙烯酰胺凝胶电泳(SDS-PAGE)研究了超声作用下三种稀土离子(La³⁺、Gd³⁺、Yb³⁺)存在的不同体系中, 纳米TiO₂与牛血清白蛋白(BSA)的相互作用. 结果表明: 在超声作用下, 不同体系中纳米TiO₂均导致BSA的内源荧光猝灭, 其猝灭机制主要为静态猝灭和非辐射能量转移; 凝胶电泳结果表明, 不同体系中的纳米TiO₂均未对BSA的结构造成明显的损伤, 由热力学数据确定其作用力主要为氢键和范德华力. 稀土离子的存在使纳米TiO₂与BSA的结合常数、结合距离和热力学参数发生改变, 但结合位点数和作用力类型基本不变. 稀土离子对纳米TiO₂与BSA的结合产生明显的“四分组效应”, 它们分别以“离子架桥”和“同位取代”方式参与纳米TiO₂与BSA的结合过程.

关键词: 超声, 纳米TiO₂, 牛血清白蛋白, 稀土离子, 结合作用

Abstract: The effects of rare earth ions (La³⁺ , Gd³⁺ , Yb³⁺ ) on the interactions between nano TiO₂ and bovine serum albumin (BSA) were investigated in the presence of ultrasound. A combination of ultraviolet (UV) spectroscopy, fluorescence spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize the interactions under simulated human physiological conditions. The endogenous fluorescence of BSA was quenched by nano TiO₂ in different systems. The mechanism of fluorescence quenching was static quenching with non-radiative energy transfer. SDS-PAGE revealed that the structure of BSA was not obviously destroyed upon binding with nano TiO₂ in different systems. Hydrogen bond and van der Waals interaction were deduced, on the basis of the thermodynamic parameters, to be the major driving forces. The binding distance of nano TiO₂ to BSA and the thermodynamic parameters were changed in the presence of rare earth ions. However, the number of binding sites and the type of intermolecular force remained essentially unchanged. This indicated that the interaction between nano TiO₂ and BSA was influenced by the rare earth ions, and that a lanthanide tetrad effect was observed. It is conjectured that rare earth ions participate in the nano TiO₂-BSA interaction process by means of“ionic bridge”formation or“appositional substitution”.

Key words: Ultrasound, Nano TiO₂, Bovine serum albumin, Rare earth ion, Interaction

高晓亚, 文雯, 宋志英, 张爱平, 郝娟, 黄茜. 超声作用下稀土离子对纳米TiO₂与牛血清白蛋白相互作用的影响[J]. 物理化学学报, 2012, 28(02), 470-478. doi: 10.3866/PKU.WHXB201112051

GAO Xiao-Ya, WEN Wen, SONG Zhi-Ying, ZHANG Ai-Ping, HAO Juan, HUANG Qian. Effects of Rare Earth Ions on the Interaction between Nano TiO₂ and Bovine Serum Albumin in the Presence of Ultrasound[J]. Acta Phys. -Chim. Sin. 2012, 28(02), 470-478. doi: 10.3866/PKU.WHXB201112051

链接本文: https://www.whxb.pku.edu.cn/CN/10.3866/PKU.WHXB201112051

https://www.whxb.pku.edu.cn/CN/Y2012/V28/I02/470

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超声作用下稀土离子对纳米TiO₂与牛血清白蛋白相互作用的影响

Effects of Rare Earth Ions on the Interaction between Nano TiO₂ and Bovine Serum Albumin in the Presence of Ultrasound

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