物理化学学报 >> 2001, Vol. 17 >> Issue (07): 582-585.doi: 10.3866/PKU.WHXB20010702

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利用分子烙印技术分离中草药活性组分

谢建春;骆宏鹏;朱丽荔;周力;李崇熙;徐筱杰   

  1. 北京大学化学与分子工程学院,　北京　100871
  • 收稿日期:2001-04-04 修回日期:2001-04-25 发布日期:2001-07-15
  • 通讯作者: 徐筱杰 E-mail:xiaojxu@chem.pku.edu.cn

Extracting Active Compounds from Herbs Using Molecular Imprinting Technology

Xie Jian-Chun;Luo Hong-Peng;Zhu Li-Li;Zhou Li;Li Chong-Xi;Xu Xiao-Jie   

  1. College of Chemistry and Molecular Engineering,Peking University,Beijing　100871
  • Received:2001-04-04 Revised:2001-04-25 Published:2001-07-15
  • Contact: Xu Xiao-Jie E-mail:xiaojxu@chem.pku.edu.cn

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摘要： 用非共价法，在极性溶剂中，以丙烯酰胺作功能单体，以强极性化合物槲皮素为模板 ，制备了分子烙印聚合物(MIP). 液相色谱实验表明, MIP 对槲皮素具有特异的亲合性.将此 MIP直接分离银杏叶提取物水解液, 得到主要含模板槲皮素及与槲皮素结构相似化合物山奈 酚两种黄酮的组分.研究证实了MIP技术用于直接分离、提取中草药中具有特定药效化合物的 可行性.

关键词: 分子烙印, 槲皮素, 山奈酚, 银杏叶, 丙烯酰胺

Abstract: A molecularly imprinted polymer (MIP) was prepared using quercetin as the template and acrylamide as the hydrogen-bonding functional monomer in a pol ar solvent. The polymer exhibited high affinity for quercetin and good selectivi ty among the tested compounds. And when it was used as an extraction cartridge f or the hydrolyzate of gingko leaves,it can purify the flavonoid component in the hydrolyzate,catching mainly quercetin and kaempferol. Thus it demonstrated th e feasibility of using MIP in extracting specific pharmacophoric compounds direc tly from herbs.

Key words: Molecularly imprinted polymer, Quercetin, Kaempferol, Gingko, Acr ylamide