物理化学学报 >> 1996, Vol. 12 >> Issue (10): 946-949.doi: 10.3866/PKU.WHXB19961019

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正交晶型江浙蝮蛇毒碱性磷脂酶A2初步结晶学研究

孟五一,牛秀田,桂璐璐,林政炯,朱洪,周元聪,李根培   

  1. 中国科学院生物物理研究所生物大分子国家实验室，北京 100101|中国科学院上海生物化学研究所，上海200032|北京大学化学系，北京 100871
  • 收稿日期:1996-03-25 修回日期:1996-06-20 发布日期:1996-10-15
  • 通讯作者: 林政炯

Cystallization and Preliminary Diffraction Data of Orthorhombic Crystal Form of Basic Phospholipase A2 from the Venom of Agkistrodon Halys Pallas

Meng Wu-Yi,Niu Xiu-Tian,Gui Lu-Lu,Lin Zheng-Jiong,Zhu Hong,Zhou Yuan-Cong,Li Gen   

  1. Natinal Laboratory of Biomacromolecules,Institute of Biophysics,Academia Sinica,Beijing 100101|Shanghai Institute of Biochemistry,Academia Sincia,Shanghai 200032;Department of Chemistry,Peking University,Beijing 100871
  • Received:1996-03-25 Revised:1996-06-20 Published:1996-10-15
  • Contact: Lin Zheng-Jiong

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关键词: 碱性磷脂酶A2, 江浙蝮蛇毒, 溶血作用, 结晶, X射线衍射

Abstract:

The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9.175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolution using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewer molecules in the asymmetric unit.

Key words: Basic phospholipase A2, Agkistrodon halys pallas, Hemolysis, Crystallization, X-ray diffraction