双光子敏化Eu3+高效发光活细胞成像纳米生物探针

doi:10.3866/PKU.WHXB201208161

物理化学学报 >> 2012, Vol. 28 >> Issue (10): 2480-2486.doi: 10.3866/PKU.WHXB201208161

双光子敏化Eu³⁺高效发光活细胞成像纳米生物探针

符小艺^1,2, 邵光胜¹, 韩荣成¹, 马严¹, 薛富民¹, 杨帆³, 付立民³, 张建平³, 王远¹

1 北京分子科学国家实验室, 分子动态与稳态国家重点实验室, 北京大学化学与分子工程学院, 北京100871;
2 华南理工大学材料科学与工程学院, 广州510640;
3 中国人民大学化学系, 北京100872

收稿日期:2012-06-20 修回日期:2012-08-16 发布日期:2012-09-26
通讯作者: 王远 E-mail:wangy@pku.edu.cn
基金资助:
国家自然科学基金(21073002, 21133001, 21227803, 51121091)及国家重点基础研究专项经费(2011CB808702)资助

Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging

FU Xiao-Yi^1,2, SHAO Guang-Sheng¹, HAN Rong-Cheng¹, MA Yan¹, XUE Fu-Min¹, YANG Fan³, FU Li-Min³, ZHANG Jian-Ping³, WANG Yuan¹

1 Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China;
2 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, P. R. China;
3 Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China

Received:2012-06-20 Revised:2012-08-16 Published:2012-09-26
Contact: WANG Yuan E-mail:wangy@pku.edu.cn
Supported by:
The project was supported by the National Natural Science Foundation of China (21073002, 21133001, 21227803, 51121091) and National Key Basic Research Special Foundation of China (2011CB808702).

摘要/Abstract

摘要：

将Eu(tta)₃dpbt (dpbt: 2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine; tta:thenoyltrifluoroacetonato)包埋在甲基丙烯酸甲酯-苯乙烯共聚物、正辛基三甲氧基硅及其水解缩合产物组成的杂化基质中, 制备了Eu(tta)₃dpbt 质量分数为40%的荧光纳米粒子, 其平均粒径为45 nm. 所制备的发光纳米粒子在水中分散稳定性高、光稳定性好、细胞毒性低、长波敏化Eu³⁺发光性能优良, 适宜作为生物分析的发光标记物. 所制备的发光纳米粒子的可见区激发峰位于415 nm, 激发峰尾部延展至475 nm, 其发光量子产率为0.31(λ_ex=415 nm, T=23 ℃), 最大双光子激发作用截面为5.0×10⁵ GM (λ_ex=830 nm, 1 GM=10^-50 cm⁴·s·photo^-1×particle^-1). 以转铁蛋白修饰上述发光纳米粒子表面制备的纳米生物探针被成功应用于活的HeLa肿瘤细胞的特异性标记和双光子激发Eu³⁺发光成像.

关键词: 发光, 铕配合物, 双光子激发, 纳米生物探针, 细胞成像

Abstract:

Luminescent nanospheres (EuPHS, d_av=45 nm) containing 40% (w) of Eu(tta)₃dpbt (tta= thenoyltrifluoroacetonato;dpbt=2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine) were prepared by encapsulating Eu(tta)₃dpbt in a hybrid matrix formed in situ from poly(styrene-co-methyl methacrylate), octyltrimethoxysilane, and poly(octylsiloxane). The EuPHS are promising luminescent markers for bioanalysis because of their good dispersibility in aqueous solutions, high photostability, low cytotoxicity, and bright Eu³⁺ luminescence under excitation at long wavelengths. EuPHS exhibited excellent visible light-sensitized and near-infrared two-photon-sensitized Eu³⁺ luminescence properties, with a visible-light excitation peak at 415 nm and an excitation window extending up to 475 nm. The quantum yield for Eu³⁺ luminescence was 0.31 (λ_ex=415 nm, T=23 ℃), and the two-photon excitation (TPE) action cross section was 5.0×10⁵ GM (1 GM=10^-50 cm⁴·s·photo^-1×particle^-1) at 830 nm. Bionanoprobes prepared by adsorbing transferrin on the surfaces of EuPHS were successfully applied in target-specific labeling and two-photon-excitation imaging of live HeLa cells.

Key words: Luminescence, Europium complex, Two-photon excitation, Bio-nanoprobe, Cell Imaging

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符小艺, 邵光胜, 韩荣成, 马严, 薛富民, 杨帆, 付立民, 张建平, 王远. 双光子敏化Eu³⁺高效发光活细胞成像纳米生物探针[J]. 物理化学学报, 2012, 28(10): 2480-2486.

FU Xiao-Yi, SHAO Guang-Sheng, HAN Rong-Cheng, MA Yan, XUE Fu-Min, YANG Fan, FU Li-Min, ZHANG Jian-Ping, WANG Yuan. Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging[J]. Acta Phys. -Chim. Sin., 2012, 28(10): 2480-2486.

链接本文: https://www.whxb.pku.edu.cn/CN/10.3866/PKU.WHXB201208161

https://www.whxb.pku.edu.cn/CN/Y2012/V28/I10/2480

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双光子敏化Eu³⁺高效发光活细胞成像纳米生物探针

Nanoprobes with Enhanced Two-Photon-Sensitized Eu³⁺ Luminescence Properties for Live Cell Imaging

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