用微量热法研究漆酶和过氧化氢的反应

doi:10.3866/PKU.WHXB19910422

Acta Phys. -Chim. Sin. ›› 1991, Vol. 7 ›› Issue (04): 490-494.doi: 10.3866/PKU.WHXB19910422

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Study on the Reaction of Laccase and Hydrogen Peroxide by Microcalorimetry

Wu Ding-Quan; Mei Fu-Ming; Qu Song-Sheng; Du Yu-Min; Zhan Dong-Feng

Department of Chemistry, Wuhan University, Wuhan 430072; Institute of Resource Chemistry, Wuhan University

Received:1990-03-07 Revised:1991-02-02 Published:1991-08-15
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Abstract

Abstract: Study on thermokinetic properties of the reaction between laccase and hydrogen peroxide by microcalorimetry has been carried out. The rate constant of the reaction is k_1=4.15×10~(-4) s~(-1). The reoxidation of the product of the reaction of laccase with hydroquinone by hydrogen peroxide obeys Michaelis-Menten's mechanism. The Michael constant K_m equals 0.0392M.

Key words: Microcalorimetry, Rate constant, Michaelis constant, Laccase, Substrate

Wu Ding-Quan; Mei Fu-Ming; Qu Song-Sheng; Du Yu-Min; Zhan Dong-Feng. Study on the Reaction of Laccase and Hydrogen Peroxide by Microcalorimetry[J]. Acta Phys. -Chim. Sin. 1991, 7(04), 490-494. doi: 10.3866/PKU.WHXB19910422

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Study on the Reaction of Laccase and Hydrogen Peroxide by Microcalorimetry

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