Acta Phys. -Chim. Sin. ›› 1996, Vol. 12 ›› Issue (05): 476-479.doi: 10.3866/PKU.WHXB19960519

• Note • Previous Articles    

Studies on Heat Denaturation and Thermodynamics of Rice Mitochondrial DNA

Zhou Pei-Jiang,Xie Chang-Li,Qu Song-Sheng,Zhou Han-Tao,Zhu Ying-Guo   

  1. Department of Chemistry,Wuhan University,Wuhan 430072|School of Life Science,Wuhan University,Wuhan 430072
  • Received:1995-09-20 Revised:1996-02-05 Published:1996-05-15
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Abstract:

The heat denaturation of the rice mitochondrial DNA in two different media has been studied by using ultraviolet absorption spectrometry over the temperature range 25-85 ℃ The heat denaturation curve of the rice mitochondrial DNA in 0.15 mol﹒L-1 NaCl solution has been obtained and the thermodynamic functions of the rice mitochondrial DNA in the heat denaturation process have been calculated. Furthermore, the relationship between these thermodynamic functions and the structure of the rice mitochondrial DNA has been discussed preliminarily.

Key words: Rice mitochondrial DNA, Heat denaturation, Ultraviolet spectrum, Thermodynamics