Acta Phys. -Chim. Sin. ›› 2001, Vol. 17 ›› Issue (01): 48-52.doi: 10.3866/PKU.WHXB20010110

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Reaction Mechanism between Ciprofloxacin Hydrochloride and Bovine Serum Albumin

Shang Zhi-Cai;Yi Ping-Gui;Yu Qing-Sen;Lin Rui-Sen   

  1. Department of chemistry,Zhejiang University,Hangzhou 310027
  • Received:2000-05-15 Revised:2000-07-25 Published:2001-01-15
  • Contact: Shang Zhi-Cai

Abstract: The reaction between ciprofloxacin and bovine serum albumin(BSA) in aqueous solution was studied by fluorescence spectra and microcalorimetry.The binding constant K was found to be 8.39×104L•mol-1 and the binding sites n to be 1.18 by fluorescence quenching method.The binding distance (r=2.46 nm) and transfer efficiency (E=0.33) between ciprofloxacin and BSA were also obtained according to the Fōrster theory of non-radiation energy transfer.Microcalorimetric measurements showed that the molar change of enthalpy ΔrHm≈0 for the reaction.The interaction between ciprofloxacin and BSA is stronger and the main binding force is hydrophobic interaction.

Key words: Ciprofloxacin, Bovine serum albumin, Fluorescence, Microcalorimetry