Abstract: A 12-residue peptide (R1G2T3F4W5V6d-P7S8V9N10Y11F12, β2) with cross-strand amino acid pairs V6V9, F4Y11 and Y3F12, predicted to form β-hairpin, was designed and synthesized by Fmoc/But strategy. The circular dichroism spectrum ofβ2 in PBS buffer shows a maximum at about 202 nm and a minimum at about 217.5 nm, a typical β-hairpin characteristics that have been postulated as the common contribution from a β-turn mixed with a β-sheet. The FT-IR experiments confirmed the secondary structural contents of β2.

Key words: de novo design, β-hairpin, β-turn, β-sheet, Cross-strand amino acid pairs, CD, FT-IR