Abstract: This paper reports the preparation and properties of biosenor using conducting composite material consisted of ethyl cellulose and acetylene black. The final biosensor was constructed by applying a mixture containing 0.010 g ethyl cellulose, 0.004 g acetylene black，a little paraffin wax and 0.2 mg glucose oxidase (23000 units/g solid, E.C.1.1.3.4, Sigma Chemical Co.) on a platinum electrode (3 mm×3 mm). Effect of various factors on the response current of the biosensor was discussed. The response current increases linearly with increasing glucose concentration under 11.5 mmol•dm－3. Its activation energy is 40.3 kJ•mol－1 in enzymecatalyzed reaction. AFM shows that the surface of conducting polymer composite materialglucose oxidase electrode′s presents uniform granular structure after paraffin wax being washed with cyclohexane, which is favorable for enzymecatalyzed reaction.

Key words: Biosensor, Conducting composite material, Glucose oxidase, Ethyl cellulose, Acetylene black