Acta Phys. -Chim. Sin. ›› 2008, Vol. 24 ›› Issue (04): 625-632.doi: 10.1016/S1872-1508(08)60029-7

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Effects of Triton X-100 on Properties of Hemoglobin and Controlled Release of Drug in Hemoglobin/Ribavirin/H2O System

LIU Tian-Qing   

  1. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 250002, Jinagsu Province, P. R. China
  • Received:2007-09-21 Revised:2007-11-29 Published:2008-04-07
  • Contact: LIU Tian-Qing E-mail:tqliu@yzu.edu.cn

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Abstract: Effects of TritonX-100 on the properties of hemoglobin (Hb) and on the controlled release of ribavirin were studied using the methods of UV-Vis spectrum, fluorescence spectrum, zeta potential, conductivity, high-performance liquid chromatographic (HPLC), and image morphology in Hb/ribavirin/H2Osystem. With the increase of concentration of Triton X-100 in the system, the intrinsic fluorescence intensity, synchronous fluorescence intensity, fluorescence polarization, zeta potential, and morphology of Hb all changed gradually, and the ribavirin located on the Hb surface was dissociated and released out. When the concentration of Triton X-100 was higher than 1×10-5 mol·L-1, the stronger interaction of Triton X-100 with Hb was predominant. Hb was unfolded and denaturized. A little Triton X-100 can protect Hb fromthe effects of ribavirin.

Key words: Hemoglobin, Property, Ribavirin, Controlled release, Triton X-100