物理化学学报 >> 2002, Vol. 18 >> Issue (08): 673-675.doi: 10.3866/PKU.WHXB20020801

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单个蓝藻细胞的光谱

应丽贞;黄旭;黄波;赵进东;赵新生   

  1. 北京大学分子动态与稳态结构国家重点实验室,化学与分子工程学院化学生物学系；北京大学生命科学学院,北京　100871
  • 收稿日期:2002-04-04 修回日期:2002-05-07 发布日期:2002-08-15
  • 通讯作者: 赵新生 E-mail:zhaoxs@chem.pku.edu.cn

Spectra of Single Cyanobacterial Cells

Ying Li-Zhen;Huang Xu;Huang Bo;Zhao Jin-Dong;Zhao Xin-Sheng   

  1. State Key Laboratory of Molecular Dynamic and Stable Structures, Department of Chemical Biology, College of Chemistry and Molecular Engineering;College of Life Sciences, Peking University, Beijing　100871
  • Received:2002-04-04 Revised:2002-05-07 Published:2002-08-15
  • Contact: Zhao Xin-Sheng E-mail:zhaoxs@chem.pku.edu.cn

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摘要： 利用共焦显微和光栅光谱技术，得到了蓝藻Anabaena strain PCC 7120完整藻丝体中的单细胞吸收和荧光发射光谱.发现了前人未观察到的不同异形胞之间有显著光谱差异的现象，该现象可能与异形胞不同发育阶段的藻胆体和光系统的演化相关联.

关键词: 蓝藻, 单个细胞, 异形胞, 共焦显微术, 吸收光谱, 荧光发射光谱

Abstract: With confocal microscopy and grating spectrograph, the absorption and fluorescence emission spectra of single Anabaena strain PCC 7120 cells have been studied in intact filaments in situ. The different spectra between vegetative cells and heterocysts are observed. The evident heterogeneities in heterocysts that have not been reported previously are attributed to the different stages of the evolution of phycobilisomes and photosystems in heterocysts.

Key words: Cyanobacteria, Single cell, Heterocyst, Confocal microscopy, 　Absorption spectra, Fluorescence emission spectra