物理化学学报 >> 2003, Vol. 19 >> Issue (02): 134-138.doi: 10.3866/PKU.WHXB20030209

研究论文 上一篇    下一篇

两种稀土卟啉配合物与大肠杆菌作用的微量热研究

侯安新;屈松生;黄伟国;刘义   

  1. 武汉大学化学与分子科学学院化学系,武汉　430072;香港浸会大学理学院化学系,香港
  • 收稿日期:2002-06-14 修回日期:2002-08-30 发布日期:2003-02-15
  • 通讯作者: 刘义 E-mail:liuyi@chem.whu.edu.cn

Microcalorimetric Study of the Action of Two Kinds of Lanthanide Monoporphyrinate Complexes on Growth Metabolism of Escherichia Coli

Hou An-Xin;Qu Song-Sheng;Wong Wai-Kwok;Liu Yi   

  1. Department of Chemistry, College of Chemistry and Molecular Science, Wuhan University, Wuhan　430072；Department of Chemistry, Hong Kong Baptist University, Hong Kong
  • Received:2002-06-14 Revised:2002-08-30 Published:2003-02-15
  • Contact: Liu Yi E-mail:liuyi@chem.whu.edu.cn

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摘要： 用LKB-2277生物活性检测系统测定了新合成的阳离子型稀土卟啉配合物{[Re(TMP)(H2O)3]Cl, Re=Y、Yb, TMP=5, 10, 15, 20-四(4’-甲氧基苯基)卟啉}在37 ℃时对大肠杆菌作用的产热曲线,根据产热曲线求算了在稀土卟啉配合物作用下,大肠杆菌生长代谢的速率常数k,抑制率I,传代时间tG和半抑制浓度cI,50等热动力学参数.结果表明,稀土卟啉配合物在低浓度下对大肠杆菌有刺激作用,高浓度下为抑制作用,[Yb(TMP)(H2O)3]Cl的半抑制浓度cI,50为143 mg•L-1,其对大肠杆菌的抑制作用优于[Y(TMP)(H2O)3]Cl.

关键词: 阳离子稀土（III）卟啉配合物, 微量热学, 大肠杆菌, 双向调节作用, 热动力学参数

Abstract: The biothermochemical characteristics of the actions of two kinds of cationic lanthanide(III) monoporphyrinate complexes {[Re(TMP)(H2O)3]Cl, Re=Y, Yb, TMP=5,10,15,20 -tetrakis(4’-methoxyphenyl)porphyrin} on growth metabolism of Escherichia coli were studied by means of a LKB-2277 bioactivity monitor at 37 ℃.The maximum power, Pmax; the growth rate constant k1,k2; inhibitive ratio I(％) and half inhibition concentration cI,50 (mg•L-1),respectively, were obtained. Experimental results show that porphyrinate complexes possess the bi-directional biological effection: they stimulate the growth of the E. coli at lower concentration but inhibit the growth at higher concentration. Inhibitory effection of [Yb(TMP)(H2O)3]Cl (cI,50=143 mg•L-1) is stronger than that of [Y(TMP)(H2O)3]Cl.

Key words: Cationic lanthanide(III) monoporphyrinate complexes, 　　Microcalorimetriy, Escherichia coli(E.coli), Bi-directional effection, 　Thermokinetic parameters