物理化学学报 >> 2004, Vol. 20 >> Issue (08): 864-867.doi: 10.3866/PKU.WHXB20040816

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荧光法研究氧氟沙星与牛乳铁蛋白的相互结合作用

商志才;范成平;郭明;俞庆森   

  1. 浙江大学化学系,杭州　310027
  • 收稿日期:2004-01-05 修回日期:2004-04-26 发布日期:2004-08-15
  • 通讯作者: 商志才 E-mail:shangzc@mail.hz.zj.cn

A Study on the Interaction between Ofloxacin and Bovine Lactoferrin by Fluorescence Spectrophotometry

Shang Zhi-Cai;Fan Cheng-Ping;Guo Ming;Yu Qing-Sen   

  1. Department of Chemistry, Zhejiang University, Hangzhou　310027
  • Received:2004-01-05 Revised:2004-04-26 Published:2004-08-15
  • Contact: Shang Zhi-Cai E-mail:shangzc@mail.hz.zj.cn

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摘要： 用荧光光谱法研究了稀水溶液中牛乳铁蛋白与氧氟沙星分子之间的结合作用机制.以荧光猝灭法测定了该反应的结合常数K, 结合位点数n；并依据Förster偶极-偶极无辐射能量转移机制,得到氧氟沙星和牛乳铁蛋白色氨酸残基之间结合距离r和能量转移效率E；并用同步荧光技术考察了氧氟沙星对牛乳铁蛋白构象的影响；牛乳铁蛋白与氧氟沙星分子之间有较强的结合作用,而且氧氟沙星对牛乳铁蛋白的构象有一定的影响.

关键词: 氧氟沙星, 牛乳铁蛋白, 荧光猝灭, 能量转移

Abstract: The binding reaction between ofloxacin and bovine lactoferrin(BLf) was studied by fluorescence spectra in dilute aqueous solution. The binding constant K was found to be 9.4×104 L•mol－1 and the binding sits n to be 2.43 by fluorescence quenching method. The binding distance (r=2.93 nm) and energy-transfer efficiency (E=0.253) between ofloxacin and bovine lactoferrin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effect of ofloxacin on the conformation of bovine lactoferrin was also analyzed using synchronous fluorescence spectroscopy. The interaction between ofloxacin and bovine lactoferrin is quite strong. Ofloxacin can effect the conformation of bovine lactoferrin in some degree.

Key words: Ofloxacin, Bovine lactoferrin, Fluorescence quenching, Energy transfer