物理化学学报 >> 2004, Vol. 20 >> Issue (08): 887-891.doi: 10.3866/PKU.WHXB20040821

研究简报 上一篇    下一篇

La3＋或Eu3＋与微过氧化物酶-8相互作用强弱的研究

陈婷婷;黄晓华;杜江燕;冯玉英;刘长鹏;邢巍;陆天虹   

  1. 南京师范大学化学与环境科学学院,南京　210097; 中国科学院长春应用化学研究所,长春　130022
  • 收稿日期:2004-01-19 修回日期:2004-04-01 发布日期:2004-08-15
  • 通讯作者: 陆天虹 E-mail:tianhonglu@263.net

Study on the Interaction Strength of La3＋ or Eu3＋ with Microperoxidase-8

Chen Ting-Ting;Huang Xiao-Hua;Du Jiang-Yan;Feng Yu-Ying;Liu Chang-Peng;Xing Wei;Lu Tian-Hong   

  1. College of Chemistry and Environment Science, Nanjing Normal University, Nanjing　210097; Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun　130022
  • Received:2004-01-19 Revised:2004-04-01 Published:2004-08-15
  • Contact: Lu Tian-Hong E-mail:tianhonglu@263.net

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摘要： 研究了La3＋或Eu3＋与微过氧化酶-8(MP-8)相互作用并比较了它们与MP-8相互作用的强弱. 紫外-可见(UV-Vis)吸收光谱和电化学的结果表明, 不论在不含NaCl或含NaCl的溶液中,一个La3＋或Eu3＋优先与MP-8分子中血红素上两个丙酸基中的羧基氧发生强的键合作用,而过量的La3＋或Eu3＋与肽链上的羰基氧发生弱的相互作用.实验结果还清楚地证明Eu3＋与MP-8的相互作用要强于La3＋与MP-8的相互作用.

关键词: 微过氧化物酶-8, La3＋, Eu3＋, 紫外-可见吸收光谱, 电化学

Abstract: The interaction of La3＋ or Eu3＋ with MP-8 was studied and the interaction strength of La3＋ or Eu3＋ with MP-8 was compared. Comparing with the UV-Vis absorption spectrum of the MP-8 solution without La3＋ or Eu3＋,the position of the Soret band for the MP-8 solution with La3＋ or Eu3＋ shifts to the red direction and the absorbance decreases. The extents of the red shift of the band position and the decrease in the absorbance for Eu3＋ in the solutions without or with NaCl are larger than that for La3＋. In 0.10 mol•L－1 NaCl solution, La3＋ or Eu3＋ could completely coordinate with carboxylate oxygen of two propionic acid groups of the heme group in an MP-8 molecule when the molar ratio of La3＋ or Eu3＋ and MP-8 is 10 and 5, respectively. In addition, the cyclic voltammetric results showed that the redox peak area of Eu3＋ decreased 20％ after adding MP-8 into the solution. These results clearly illustrated that one La3＋ or Eu3＋ ion would coordinate strongly with carboxylate oxygen of two propionic acid groups of the heme group in an MP-8 molecule and the excessive La3＋ or Eu3＋ ions weakly interact with the carboxylate oxygen in the peptide chain. The interaction between Eu3＋ and MP-8 is stronger than that between La3＋ and MP-8.

Key words: Microperoxidase-8(MP-8), La3＋, Eu3＋, UV-Vis absorption spectroscopy, Electrochemistry