物理化学学报 >> 2004, Vol. 20 >> Issue (04): 386-390.doi: 10.3866/PKU.WHXB20040411

研究论文 上一篇    下一篇

羧甲基纤维素水凝胶生物降解动力学研究

聂华荣;柳明珠;陈振斌   

  1. 兰州大学化学化工学院,应用有机化学国家重点实验室,兰州　730000;河西学院化学系,张掖　734000
  • 收稿日期:2003-10-06 修回日期:2003-12-15 发布日期:2004-04-15
  • 通讯作者: 柳明珠 E-mail:m-zliu@163.com

Kinetic Study on Bio-degradation of Carboxymethylcellulose Hydrogel

Nie Hua-Rong;Liu Ming-Zhu;Chen Zhen-Bin   

  1. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou　730000;Department of Chemistry, Hexi College, Zhangye　734000
  • Received:2003-10-06 Revised:2003-12-15 Published:2004-04-15
  • Contact: Liu Ming-Zhu E-mail:m-zliu@163.com

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摘要： 用氯化铝对羧甲基纤维素进行交联,制得了水凝胶.考察了底物浓度、酶浓度以及降解温度对该水凝胶降解速率的影响,探讨了酶降解动力学及“表观”活化能对酶浓度的依赖关系.结果表明,该酶促反应最佳温度为37 ℃,降解反应对底物浓度和酶浓度的反应级数分别为1级和1.2级;得到了与传统的Michaelis-Menten动力学机制不同的非均相酶促反应动力学模型,确定了“表观”活化能与酶浓度之间的定量关系.

关键词: 羧甲基纤维素(CMC), 水凝胶, 生物降解, 动力学

Abstract: Carboxymethylcellulose(CMC) hydrogels have attracted attention as biodegradation absorbent materials in agriculture and forestry. It is important to investigate the biodegradation behavior and kinetics of the bio-degradable reaction. In this paper, CMC hydrogel was prepared by crosslinking with aluminum chloride. The effects of the substrate concentration, enzyme concentration and reaction temperature on the degradation rates were investigated. At the same time, the kinetics of the enzymatic degradation and the relationship between the apparent-activation energy and enzyme concentration were also discussed in detail. The results showed that the optimal temperature for the enzymatic reaction was 37 ℃.The reaction orders for the substrate and enzyme were 1 and 1.2, respectively. A new kinetic model for CMC hydrogel heterogeneous degradation was proposed which was different from the traditional Michaelis-Menten kinetics. The relationship between the apparent-activation energy and the enzyme concentration was also established.

Key words: Carboxymethylcellulose(CMC), Hydrogel, Bio-degradation, Kinetics