物理化学学报 >> 2010, Vol. 26 >> Issue (04): 1119-1123.doi: 10.3866/PKU.WHXB20100427

生物物理化学 上一篇    下一篇

寡聚酰胺为探针的电化学DNA生物传感器

曹瑞国, 朱斌, 刘巍, 李经建, 徐东升   

  1. 北京大学化学与分子工程学院, 分子动态与稳态结构国家重点实验室, 北京分子科学国家实验室, 北京 100871
  • 收稿日期:2009-12-11 修回日期:2010-01-26 发布日期:2010-04-02
  • 通讯作者: 李经建 E-mail:lijj@pku.edu.cn

Electrochemical DNA Biosensor with a Polyamide Probe

CAO Rui-Guo, ZHU Bin, LIU Wei, LI Jing-Jian, XU Dong-Sheng   

  1. Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China
  • Received:2009-12-11 Revised:2010-01-26 Published:2010-04-02
  • Contact: LI Jing-Jian E-mail:lijj@pku.edu.cn

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摘要：

DNA分子中的碱基对可以长程传递电荷, DNA分子中的碱基π堆积结构为电荷的长程传递提供了良好的通道. 电荷在DNA分子中的传递受碱基序列的影响, 利用这种性质可以构建DNA碱基错配检测的电化学传感器. 寡聚酰胺能和DNA以小沟绑定方式高亲和力地结合, 并且具有序列识别功能, 本文以带有硝基官能团的寡聚酰胺分子为电化学探针, 设计了电化学DNA生物传感器. 结果显示, 寡聚酰胺与DNA修饰电极作用后, 电化学响应显著增强, 并且可以作为检测DNA碱基错配的电化学探针分子.

关键词: 生物传感器, 电化学, 寡聚酰胺, DNA, 碱基错配

Abstract:

The base pair stack within a DNA duplex can transport charge over long distances. The π-stacked base pairs provide an effective medium for long distance charge transport in DNA molecules. The DNA-mediated charge transport is extremely sensitive to the DNA sequence. This sensitivity can be used to fabricate electrochemical DNA biosensors for single base mismatch sensing. Polyamides are DNA recognition molecules which can bind to the minor groove in DNA to form a DNA/polyamide complex with high affinity and specificity. Polyamide molecules with the nitro group are used as an electrochemically active probe for DNA biosensors. The electrochemical properties of the nitro-polyamide was investigated on a DNA self-assembly electrode. The results indicated that the electrochemical response was enhanced intensitively, and the polyamide can be used as a probe to detect a single base pair mismatch in DNA.

Key words: Biosensor, Electrochemistry, Polyamide, DNA, Mismatch