聚甲基丙烯酸甲酯-牛血清白蛋白核壳纳米粒子在金表面的吸附过程及在传感器中的应用

doi:10.3866/PKU.WHXB201207191

Abstract

Abstract:

Core-shell poly(methyl methacrylate)-bovine serum albumin (PMMA-BSA) nanoparticles with PMMA cores and BSA shells were prepared via a copper ion-mediated initiation system. The core-shell structure of the nanoparticles was characterized by transmission electron microscopy (TEM) and the surface compositions of the nanoparticles were tested by X-ray photoelectron spectroscopy (XPS), which further demonstrated that the particles have a BSA protein shell. The adsorption of these PMMA-BSA particles onto gold surfaces was studied by quartz crystal microbalance with dissipation (QCM-D). The significantly change of frequency shift and dissipation factor indicated that PMMA-BSA particles are adsorbed on the gold surface. The repeated buffer washing had nearly no effect on either frequency shift or dissipation factor, revealing that the adsorption is fairly strong. An amperometric glucose biosensor was constructed by immobilizing glucose oxidase on PMMA-BSA particles modified with glutaraldehyde, using a gold electrode as a substrate on which to adsorb the PMMA-BSA particles. Electrochemical measurements show this biosensor exhibited a good current response to glucose. Working at 0.3 V, the biosensor had a short response time of 11 s, a sensitivity of 28.6 μA·L^-1·mmol^-1·cm^-2 and a linear range from 0.2 to 5.85 mmol·L^-1 with a correlation coefficient of 0.989. After storage at 25℃ for one month, the sensor response current decreased by only 16%, thus showing good thermal stability.

Key words: Glucose oxidase, Biosensor, Quartz crystal microbalance, Frequency, Dissipation, Adsorption

HE Chuan-Xin, YUAN An-Peng, ZHANG Qian-Lin, REN Xiang-Zhong, LI Cui-Hua, LIU Jian-Hong. Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on Gold Surface and Its Sensor Application[J]. Acta Phys. -Chim. Sin. 2012, 28(11), 2721-2728. doi: 10.3866/PKU.WHXB201207191

References

(1) Wang, J. Chem. Rev. 2008, 108, 814. doi: 10.1021/cr068123a
(2) Koschwanez, H. E.; Reichert,W. M. Biomaterials 2007, 28,3687. doi: 10.1016/j.biomaterials.2007.03.034
(3) Hu, J. Biosens. Bioelectron. 2009, 24, 1083. doi: 10.1016/j.bios.2008.08.051
(4) Ge, F.; Cao, R. G.; Zhu, B.; Li, J. J.; Xu, D. S. Acta Phys. -Chim. Sin. 2010, 26, 1779. [戈芳, 曹瑞国, 朱斌, 李经建, 徐东升. 物理化学学报, 2010, 26, 1779.] doi: 10.3866/PKU.WHXB20100736
(5) Willner, I.; Baron, R.;Willner, B. Biosens. Bioelectron. 2007,22, 1841. doi: 10.1016/j.bios.2006.09.018
(6) Zargoosh, K.; Chaichi, M. J.; Shamsipur, M.; Hossienkhani, S.;Asghari, S.; Qandalee, M. Talanta 2012, 93, 37. doi: 10.1016/j.talanta.2011.11.029
(7) Xiao, X.; Zhou, B.; Zhu, L.; Xu, L.; Tan, L.; Tang, H.; Zhang,Y.; Xie, Q.; Yao, S. Sens. Actuators B 2012, 165, 126. doi: 10.1016/j.snb.2012.02.029
(8) Willner, I.;Willner, B.; Katz, E. Bioelectrochemistry 2007, 70,2. doi: 10.1016/j.bioelechem.2006.03.013
(9) Heller, A.; Feldman, B. Chem. Rev. 2008, 108, 2482. doi: 10.1021/cr068069y
(10) Guo, X. L.; Guo, M.;Wang, X. D. Acta Phys. -Chim. Sin. 2007,23, 585. [郭小丽, 郭敏, 王新东. 物理化学学报, 2007, 23,585.] doi: 10.3866/PKU.WHXB20070426
(11) Cui, G.; Kim, S. J.; Choi, S. H.; Nam, H.; Cha, G. S.; Paeng, K.J. Anal. Chem. 2000, 72, 1925. doi: 10.1021/ac991213d
(12) Weibel, M. K.; Bright, H. J. J. Biol. Chem. 1971, 246, 2743.
(13) Wrighton, M. S. Science 1986, 231, 32. doi: 10.1126/science.231.4733.32
(14) Frederick, K. R.; Tung, J.; Emerick, R. S.; Masiarz, F. R.;Chamberlain, S. H.; Vasavada, A.; Rosenberg, S.; Chakraborty,S.; Schopter, L. M.; Massey, V. J. Biol. Chem. 1990, 265, 3793.
(15) Hecht, H. J.; Schomburg, D.; Kalisz, H.; Schmid, R. D. Biosens. Bioelectron. 1993, 8, 197. doi: 10.1016/0956-5663(93)85033-K
(16) Yu, J.; Yu, D.; Zhao, T.; Zeng, B. Talanta 2008, 74, 1586. doi: 10.1016/j.talanta.2007.10.005
(17) Deng, S.; Jian, G.; Lei, J.; Hu, Z.; Ju, H. Biosens. Bioelectron.2009, 25, 373. doi: 10.1016/j.bios.2009.07.016
(18) Wei, Y.; Li, Y.; Liu, X.; Xian, Y.; Shi, G.; Jin, L. Biosens. Bioelectron. 2010, 26, 275. doi: 10.1016/j.bios.2010.06.006
(19) Zhang, G. L.; Pan, X. H.; Kan, J. Q.; Zhang, J. H.; Li, Y. F. Acta Phys. -Chim. Sin. 2003, 19, 533. [张国林, 潘献华, 阚锦晴,张景辉, 李永舫. 物理化学学报, 2003, 19, 533.] doi: 10.3866/PKU.WHXB20030611
(20) Qiu, J. D.; Huang, J.; Liang, R. P. Sens. Actuators B 2011, 160,287. doi: 10.1016/j.snb.2011.07.049
(21) Chen, X.; Zhu, J.; Chen, Z.; Xu, C.;Wang, Y.; Yao, C. Sens. Actuators B 2011, 159, 220. doi: 10.1016/j.snb.2011.06.076
(22) Che, X.; Yuan, R.; Chai, Y.; Li, J.; Song, Z.; Li,W.; Zhong, X.Colloids Surf. B 2011, 84, 454. doi: 10.1016/j.colsurfb.2011.01.041
(23) Valstar, A.; Vasilescu, M.; Vigouroux, C.; Stilbs, P.; Almgren,M. Langmuir 2001, 17, 3208. doi: 10.1021/la0016221
(24) Rodahl, M.; Höök, F.; Krozer, A.; Kasemo, B.; Breszinsky, P.Rev. Sci. Instrum. 1995, 66, 3924. doi: 10.1063/1.1145396
(25) Voinova, M. V.; Rodahl, M.; Jonson, M.; Kasemo, B. Phys. Scrip. 1999, 59, 31.
(26) Bloomfield, V. Biochemistry 1965, 5, 684.
(27) Hirayama, K.; Akashi, S.; Furuya, M.; Fukuhara, K. L.Biochem. Biophys. Res. Commun. 1990, 173, 639. doi: 10.1016/S0006-291X(05)80083-X
(28) Bos, O. J. M.; Labro, J. F. A.; Fischer, M. J. E.;Wilting, J.;Janssen, L. H. M. J. Biol. Chem. 1989, 264, 953.
(29) Bontempo, D.; Heredia, K. L.; Fish, B. A.; Maynard, H. D.J. Am. Chem. Soc. 2004, 126, 15372. doi: 10.1021/ja045063m
(30) Heredia, K. L.; Bontempo, D.; Ly, T.; Byers, J. T.; Halstenberg,S.; Maynard, H. D. J. Am. Chem. Soc. 2005, 127, 16955. doi: 10.1021/ja054482w
(31) Liu, J.; Bulmus, V.; Herlambang, D. L.; Barner-Kowollik, C.;Stenzel, M. H.; Davis, T. P. Angew. Chem. Int. Edit. 2007, 46,3099.
(32) Boyer, C.; Bulmus, V.; Liu, J.; Davis, T. P.; Stenzel, M. H.;Barner-Kowollik, C. J. Am. Chem. Soc. 2007, 129, 7145. doi: 10.1021/ja070956a
(33) Cascâo Pereira, L. G.; Théodoly, O.; Blanch, H.W.; Radke, C. J.Langmuir 2003, 19, 2349. doi: 10.1021/la020720e
(34) Zhu,W.; Kapteijn, F.; Moulijn, J. A.; den Exter, M. C.; Jansen,J. C. Langmuir 2000, 16, 3322. doi: 10.1021/la9914007
(35) Wassell, D. T.; Hall, R. C.; Embery, G. Biomaterials 1995, 16,697. doi: 10.1016/0142-9612(95)99697-K
(36) Rabe, M.; Verdes, D.; Zimmermann, J.; Seeger, S. J. Phys. Chem. B 2008, 112, 13971. doi: 10.1021/jp804532v
(37) Zhu, H.; Srivastava, R.; Brown, J. Q.; McShane, M. J.Bioconjugate Chem. 2005, 16, 1451. doi: 10.1021/bc050171z
(38) Shu, F. R.;Wilson, G. S. Anal. Chem. 1976, 48, 1679. doi: 10.1021/ac50006a014
(39) Kamin, R. A.;Wilson, G. S. Anal. Chem. 1980, 52, 1198. doi: 10.1021/ac50058a010

[1]	Xiaoyu Wang, Yang Cheng, Guodong Xue, Ziqi Zhou, Mengze Zhao, Chaojie Ma, Jin Xie, Guangjie Yao, Hao Hong, Xu Zhou, Kaihui Liu, Zhongfan Liu. Giant Enhancement of Optical Second Harmonic Generation in Hollow-Core Fiber Integrated with GaSe Nanoflakes [J]. Acta Phys. -Chim. Sin., 2023, 39(7): 2212028-0.
[2]	Kangning Zhao, Xiao Li, Dong Su. High-Entropy Alloy Nanocatalysts for Electrocatalysis [J]. Acta Phys. -Chim. Sin., 2021, 37(7): 2009077-.
[3]	Jianchuan Sun, Xuhui Wang, Shuaiqi Chen, Yanqing Liao, Awang Gao, Yuhao Hu, Tao Yang, Xiangyu Xu, Yingxia Wang, Jiaqing Song. Defluoridation of Water Using Active Alumina Derived from Single-Layer Boehmite [J]. Acta Phys. -Chim. Sin., 2021, 37(10): 1911009-.
[4]	Wenyuan Wang, Jiefu Zhang, Zhe Li, Xiang Shao. Atomic Structure and Adsorption Property of the Singly Dispersed Au/Cu(111) Surface Alloy [J]. Acta Physico-Chimica Sinica, 2020, 36(8): 1911035-.
[5]	Guanqing Sun, Zonglin Yi, To Ngai. Particle-Stabilized Interfaces and Their Interactions at Interfaces [J]. Acta Physico-Chimica Sinica, 2020, 36(10): 1910005-.
[6]	Chengcheng Zhang, Ralph Crisci, Zhan Chen. Probing Molecular Structures of Antifouling Polymer/Liquid Interfaces In Situ [J]. Acta Physico-Chimica Sinica, 2020, 36(10): 1910003-.
[7]	Mengru HAN,Yanan ZHOU,Xuan ZHOU,Wei CHU. Tunable Reactivity of MNi₁₂ (M = Fe, Co, Cu, Zn) Nanoparticles Supported on Graphitic Carbon Nitride in Methanation [J]. Acta Physico-Chimica Sinica, 2019, 35(8): 850-857.
[8]	Zhe JIANG,Fei YU,Jie MA. Design of Graphene-based Adsorbents and Its Removal of Antibiotics in Aqueous Solution [J]. Acta Phys. -Chim. Sin., 2019, 35(7): 709-724.
[9]	Yan HE,Hao LI,Li ZHOU,Ting XU,Changjun PENG,Honglai LIU. Removal of Methyl Orange from Aqueous Solutions by a Novel Hyper-Cross-Linked Aromatic Triazine Porous Polymer [J]. Acta Physico-Chimica Sinica, 2019, 35(3): 299-306.
[10]	Ruilong YANG,Diyu ZHANG,Kangwei ZHU,Huanlin ZHOU,Xiaoqiu YE,Aart W. KLEYN,Yin HU,Qiang HUANG. In Situ Study of the Conversion Reaction of CO₂ and CO₂-H₂ Mixtures in Radio Frequency Discharge Plasma [J]. Acta Phys. -Chim. Sin., 2019, 35(3): 292-298.
[11]	Yuxing GU,Juan YANG,Dihua WANG. Electrochemical Features of Carbon Prepared by Molten Salt Electro-Reduction of CO₂ [J]. Acta Phys. -Chim. Sin., 2019, 35(2): 208-214.
[12]	Jyotirmoy DEB,Debolina PAUL,David PEGU,Utpal SARKAR. Adsorption of Hydrazoic Acid on Pristine Graphyne Sheet: A Computational Study [J]. Acta Phys. -Chim. Sin., 2018, 34(5): 537-542.
[13]	Xuanjun WU,Lei LI,Liang PENG,Yetong WANG,Weiquan CAI. Effect of Coordinatively Unsaturated Metal Sites in Porous Aromatic Frameworks on Hydrogen Storage Capacity [J]. Acta Phys. -Chim. Sin., 2018, 34(3): 286-295.
[14]	Yuan DUAN,Mingshu CHEN,Huilin WAN. Adsorption and Activation of O₂ and CO on the Ni(111) Surface [J]. Acta Phys. -Chim. Sin., 2018, 34(12): 1358-1365.
[15]	Qiang LIU,Yong HAN,Yunjun CAO,Xiaobao LI,Wugen HUANG,Yi YU,Fan YANG,Xinhe BAO,Yimin LI,Zhi LIU. In-situ APXPS and STM Study of the Activation of H₂ on ZnO(10${\rm{\bar 1}}$0) Surface [J]. Acta Phys. -Chim. Sin., 2018, 34(12): 1366-1372.

Adsorption of Core-Shell Poly(Methyl Methacrylate)-Bovine Serum Albumin Nanoparticles on Gold Surface and Its Sensor Application

PDF

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Recommended 0