Acta Phys. -Chim. Sin. ›› 2014, Vol. 30 ›› Issue (1): 121-128.doi: 10.3866/PKU.WHXB201311272

• SOFT MATTER • Previous Articles     Next Articles

Preparation of Erythromycin-Imprinted Polymeric Microspheres by Emulsion Polymerization and Their Adsorption Properties

ZHAO Na, HU Xiao-Ling, GUAN Ping, SONG Ren-Yuan, TIAN Tian, ZHANG Xiang-Rong   

  1. The Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, School of Natural and Applied Science, Northwestern Polytechnical University, Xi'an 710072, P. R. China
  • Received:2013-09-18 Revised:2013-11-25 Published:2014-01-01
  • Contact: ZHAO Na
  • Supported by:

    The project was supported by the National Natural Science Foundation of China (21174111).


Uniform molecularly imprinted polymeric microspheres (EM-MIPMs) were prepared by emulsion polymerization using erythromycin as the template molecule, methacrylic acid (MAA) as the functional monomer, and ethylene glycol dimethacrylate (EDMA) and sodium dodecylbenzene sulfonate (SBS) as the cross-linker and emulsifier, respectively. The obtained erythromycin-MAA complexes were characterized using ultraviolet (UV) absorption spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and 1H nuclear magnetic resonance (NMR) spectroscopy. The results showed that erythromycin-MAA complexes were obtained by cooperative hydrogen-bonding interactions. The surface features and thermal stability of the EM-MIPMs were investigated using scanning electron microscopy (SEM) and thermal gravimetric analysis (TGA). The average diameter of the EM-MIPMs was 4.24 μm, larger than non-imprinted polymeric microspheres. They exhibited excellent thermal stability. Kinetic, equilibrium adsorption, and selectivity adsorption experiments (solid-phase extraction) were used to evaluate the binding properties and molecule recognition characteristics of EM-MIPMs for erythromycin. The experimental kinetic data were well described by a pseudo-second-order kinetic model. Erythromycin binding was examined using the Langmuir and Freundlich isotherm models. The EM-MIPMs had an excellent affinity for erythromycin. The equilibrium experimental data for the EM-MIPMs fitted the Langmuir isotherm well, and the binding amount reached 0.242 mmol·g-1. Furthermore, solid-phase extraction experiments demonstrated that the EM-MIPMs had a higher affinity for the target molecules than for roxithromycin and erythromycin ethylsuccinate.

Key words: Erythromycin, Molecularly imprinted microsphere, Adsorption, Binding property, Recognition property