免刺激硒化钼量子点滴眼液杀菌抑炎协同干预真菌性角膜炎

doi:10.1016/j.actphy.2026.100373

物理化学学报

免刺激硒化钼量子点滴眼液杀菌抑炎协同干预真菌性角膜炎

耿熙炆¹, 储丹丹¹, 谷歆雨⁴, 张驰茵⁴, 潘湘蓉², 苏雨蒙², 栗占荣¹, 周战², 谭超良³, 李景果^1,4

1 河南省立眼科医院, 河南省人民医院, 郑州大学人民医院, 河南郑州 450003;
2 洛阳师范学院化学化工学院, 河南洛阳 471934;
3 香港城市大学电气工程系, 香港 999077;
4 郑州大学材料科学与工程学院, 河南郑州 450001

收稿日期:2026-04-27 修回日期:2026-07-24 录用日期:2026-07-25
通讯作者: 栗占荣, 周战, 谭超良, 李景果 E-mail:lizhanrong@zzu.edu.cn;zhouzhan@lynu.edu.cn;chaoltan@cityu.edu.hk;lijingguo@zzu.edu.cn
基金资助:
感谢河南省自然科学基金(242300421018、252300421044和252300421024)、国家自然科学基金(82371108和52173143)、河南省科技研发计划联合基金项目(242301420010)、河南省医学科技人才海外研修项目(HNMOT2024082)、河南省青年卫生健康科技创新人才培养项目(LJRC2024003)、河南省临床医学科学家培养计划(HNCMS202402)、香港城市大学启动基金(9610710)和国家贵金属材料工程技术研究中心香港分中心的资助。

Stimuli-free bifunctional MoSe₂ quantum dots exert antifungal activity and mitigate inflammation for synergistic intervening fungal keratitis

Xiwen Geng¹, Dandan Chu¹, Xinyu Gu⁴, Chiyin Zhang⁴, Xiangrong Pan², Yumeng Su², Zhanrong Li¹, Zhan Zhou², Chaoliang Tan³, Jingguo Li^1,4

1 Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, Henan Province, China;
2 College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471934, Henan Province, China;
3 Department Electrical Engineering, Department Biomedical Engineering and Hong Kong Branch of National Precious Metals Material Engineering Research Center (NPMM), City University of Hong Kong, Hong Kong, China;
4 School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China

Received:2026-04-27 Revised:2026-07-24 Accepted:2026-07-25
Contact: Zhanrong Li, Zhan Zhou, Chaoliang Tan, Jingguo Li E-mail:lizhanrong@zzu.edu.cn;zhouzhan@lynu.edu.cn;chaoltan@cityu.edu.hk;lijingguo@zzu.edu.cn

1. ACTPHY-2026.100373 (00274)-SI-23p-9#排.pdf(838KB)

摘要/Abstract

摘要： 真菌性角膜炎(FK)的有效治疗迫切需要创新策略，将强效抗真菌活性与显著的抗炎作用相结合，以克服日益严重的真菌耐药性和角膜穿透障碍，从而预防角膜纤维化和视力丧失。在此，我们开发了一种超小尺寸的二硒化钼(MoSe₂)量子点滴眼液(MSQD)，作为一种无刺激的双功能平台。MSQD对腐皮镰刀菌的最低抑菌浓度(MIC)低至0.05 μg mL^-1，比传统一线药物伏立康唑(Vor，MIC 11.76 μg mL^-1)低235倍。MSQD表现出快速且完全的杀菌活性，可以通过诱导细胞内氧化还原崩溃和不可逆的膜损伤，在1 h内实现100%的病原菌清除。此外，MSQD自身具有类过氧化氢酶(CAT)催化活性，能有效清除细胞内活性氧，减轻炎症，并形成促进愈合的角膜微环境。同时，MSQD的量子尺寸可以通过短暂性地调节细胞连接促进深层角膜穿透，从而确保其被高效递送至感染部位。在小鼠FK模型中，经MSQD治疗的眼睛实现了病原体的完全清除，并恢复了与健康组织无异的角膜透明度，与Vor相比表现出更高的生物利用度和优越的治疗效果。总之，这些多方面的特性使无刺激的MSQD成为一种新型、高效且安全的抗菌抗炎双功能纳米平台，兼具卓越的效力和优异的生物相容性，为真菌感染的综合治疗带来了巨大前景。

关键词: MoSe₂量子点, 滴眼液, 真菌性角膜炎, 缓解炎症, 量子限制效应

Abstract: Effective management of fungal keratitis (FK) urgently demands innovative strategies integrating potent antifungal activity with robust anti-inflammatory effects to overcome rising antifungal resistance and corneal penetration barriers, preventing corneal fibrosis and vision loss. Herein, we develop an ultrasmall molybdenum diselenide (MoSe₂) quantum dot eye drop (MSQD) as a stimuli-free bifunctional platform. The MSQD exhibits an ultra-low minimum inhibitory concentration (MIC) of 0.05 μg mL^-1 against Fusarium solani, approximately 235-fold lower than the conventional first-line agent voriconazole (Vor, MIC 11.76 μg mL^-1). The MSQD demonstrates rapid and complete fungicidal activity, achieving 100% cell elimination within 1 h by inducing intracellular redox collapse and irreversible membrane damage. Furthermore, the MSQD possesses intrinsic catalase (CAT)-mimetic activities, efficiently scavenging intracellular reactive oxygen species, alleviating inflammation, and promoting a pro-healing corneal microenvironment. In addition, the quantum dimensions of MSQD facilitate deep corneal penetration through transient cell junction modulation, thus ensuring efficient delivery to the infection sites. In a mouse FK model, MSQD-treated eyes achieve complete pathogen eradication and restore corneal clarity indistinguishable from healthy tissue, exhibiting great bioavailability and a superior therapeutic effect compared to Vor. Collectively, these multifaceted attributes establish stimuli-free MSQD as a novel, highly efficient and safe bifunctional nanoplatform with superior potency and excellent biocompatibility, holding great promise for comprehensive management of fungal infections.

Key words: MoSe₂ quantum dots, Eye drops, Fungal keratitis, Inflammation eliminating, Quantum confinement

耿熙炆, 储丹丹, 谷歆雨, 张驰茵, 潘湘蓉, 苏雨蒙, 栗占荣, 周战, 谭超良, 李景果. 免刺激硒化钼量子点滴眼液杀菌抑炎协同干预真菌性角膜炎[J]. 物理化学学报 2026, (), 100373. doi: 10.1016/j.actphy.2026.100373

Xiwen Geng, Dandan Chu, Xinyu Gu, Chiyin Zhang, Xiangrong Pan, Yumeng Su, Zhanrong Li, Zhan Zhou, Chaoliang Tan, Jingguo Li. Stimuli-free bifunctional MoSe₂ quantum dots exert antifungal activity and mitigate inflammation for synergistic intervening fungal keratitis[J]. Acta Phys. -Chim. Sin. 2026, (), 100373. doi: 10.1016/j.actphy.2026.100373

链接本文: https://www.whxb.pku.edu.cn/CN/10.1016/j.actphy.2026.100373

https://www.whxb.pku.edu.cn/CN/Y2026/V/I/100373

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