物理化学学报 >> 2007, Vol. 23 >> Issue (07): 1053-1058.doi: 10.3866/PKU.WHXB20070717

研究论文 上一篇    下一篇

葡萄糖氧化酶在炭黑上的固定及直接电化学

马国仙; 仲慧; 陆天虹; 夏永姚   

  1. 复旦大学化学系, 上海 200433; 淮阴师范学院化学系, 江苏 淮安 223300; 南京师范大学化学与环境科学学院, 南京 210097
  • 收稿日期:2007-01-02 修回日期:2007-03-13 发布日期:2007-07-03
  • 通讯作者: 夏永姚 E-mail:yyxia@fudan.edu.cn

Direct Electrochemistry and Immobilization of Glucose Oxidase on Carbon Black

MA Guo-Xian; ZHONG Hui; LU Tian-Hong; XIA Yong-Yao   

  1. Department of Chemistry, Fudan University, Shanghai 200433, P. R. China; Department of Chemistry, Huaiyin Teachers College, Huaian 223300, Jiangsu Province, P. R. China; School of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, P. R. China
  • Received:2007-01-02 Revised:2007-03-13 Published:2007-07-03
  • Contact: XIA Yong-Yao E-mail:yyxia@fudan.edu.cn

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摘要： 经红外光谱和电化学测量证明, 用简单的吸附法能将葡萄糖氧化酶(GOx)固定在炭黑(CB)表面. 电化学测量表明, 固定在CB上的GOx能进行准可逆的直接电化学反应, 其式量电位(E0’)为-0.436 V, 在40-150 mV·s-1范围内, 不随扫描速率而变化. 电化学反应速率常数(ks)为0.800 s-1, 比文献报道的大30多倍. 而且, 固定在CB上的GOx能保持其对葡萄糖氧化的生物电催化活性. 即使在保存两周后, 其电催化活性仅下降了5%, 表明固定在CB上的GOx有良好的稳定性.

关键词: 葡萄糖氧化酶, 炭黑, 葡萄糖, 直接电化学, 生物燃料电池

Abstract: The IR spectroscopic and electrochemical measurements demonstrated that glucose oxidase (GOx) could be immobilized on the surface of carbon black (CB) using a simple adsorptionmethod. The electrochemical measurements indicated that GOx immobilized on CB could undergo the quasi-reversible and direct electrochemical reaction and keep the bioelectrocatalytic activity for the glucose oxidation. Even after conservation for two weeks, its electrocatalytic activity decreased only 5%, illustrating the good stability of GOx immobilized on CB.

Key words: Glucose oxidase, Carbon black, Glucose, Direct electrochemistry, Biofuel cell