物理化学学报 >> 1997, Vol. 13 >> Issue (03): 236-241.doi: 10.3866/PKU.WHXB19970310

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茶碱对聚苯胺尿酸酶电极的生物电化学活性的抑制

阚锦晴,乔月东,穆绍林,李永舫   

  1. 扬州大学师范学院化学系，扬州 225002|中国科学院化学研究所，北京 100080
  • 收稿日期:1996-09-09 修回日期:1996-11-21 发布日期:1997-03-15
  • 通讯作者:

Inhibition of Bioelectrochemical Activities of Polyaniline Uricase Electrode by Theophylline

Kan Jin-Qing,Qiao Yue-Dong,Mu Shao-Lin,Li Yong-Fang   

  1. Department of Chemistry,Teacher's College,Yangzhou University,Yangzhou 225002|Institute of Chemistry,Chinese Academy,Beijing 100080
  • Received:1996-09-09 Revised:1996-11-21 Published:1997-03-15
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摘要：

用聚苯胺尿酸酶电极研究了茶碱对固定酶的影响.结果表明，茶碱对固定尿酸酶有明显的抑制作用，但这种抑制作用是可逆的.在茶碱的存在下，pH对酶电极的响应电流影响与无茶碱存在时不同；在0.2－0.5V之间，酶电极的响应电流随电位增加而迅速升高，当电位进一步升高时，其变化速率减慢.茶碱使尿酸酶催化反应的活化能从无抑制剂存在时的29.9kJ?mol-1提高到47.8kJ?mol-1，即抑制剂改变了尿酸酶催化反应的途径.

关键词: 聚苯胺, 尿酸酶, 茶碱, 抑制作用, 活化能

Abstract:

 The effect of theophylline on the immobilized enzyme has been investigated using polyaniline uricase electrode. The activity of the immobilized uricase is markedly inhibited by theophylline. However, this is a reversible inhibition. In the presence of theophylline, the effect of pH on the response current of the enzyme electrode is different from that without theophylline; the response current of the enzyme electrode rapidly increase with increasing potential from 0.20-0.50V, and then increases slowly with further increasing potential. The activation energy of the enzyme-catalyzed reaction is 47.8kJ?mol-1 in the presence of theophylline, which is much larger than that without theophylline (29.9kJ?mol-1). This indicates that the path of the enzyme-catalyzed reaction was changed by theophylline.

Key words: Polyaniline, Uricase, Theophylline, Inhibition, Activation energy