物理化学学报 >> 2007, Vol. 23 >> Issue (08): 1201-1206.doi: 10.3866/PKU.WHXB20070812

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镀金和碳纳米管修饰金电极上吸附态葡萄糖氧化酶比活性的EQCM研究

陈昕; 张漪丽; 苏育华; 孟文华; 谢青季; 姚守拙   

  1. 湖南师范大学化学化工学院, 化学生物学及中药分析省部共建教育部重点实验室, 长沙 410081; 湖南师范大学医学院, 长沙 410006; 湖南师范大学医院, 长沙 410081

  • 收稿日期:2007-02-05 修回日期:2007-04-07 发布日期:2007-08-03
  • 通讯作者: 谢青季 E-mail:xieqj@hunnu.edu.cn

EQCM Studies on Enzymatic Specific Activities of Glucose Oxidase Adsorbed on Au-electroplated and Carbon Nanotubes-modified Au Electrodes

CHEN Xin; ZHANG Yi-Li; SU Yu-Hua; MENG Wen-Hua; XIE Qing-Ji; YAO Shou-Zhuo   

  1. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China; Medical College, Hunan Normal University, Changsha 410006, P. R. China; Hunan Normal University Hospital, Changsha 410081, P. R. China

  • Received:2007-02-05 Revised:2007-04-07 Published:2007-08-03
  • Contact: XIE Qing-Ji; E-mail:xieqj@hunnu.edu.cn

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摘要： 采用石英晶体微天平(EQCM)技术监测了裸金电极、镀金和碳纳米管修饰金电极上葡萄糖氧化酶(GOD)的吸附过程. 通过EQCM测量吸附固定的GOD质量, 并实时检测酶反应产物H2O2的氧化电量, 求算了各表面上吸附态GOD的比活性(ESAi). 结果表明, 各表面上均可吸附一定的GOD, 且吸附态GOD均有一定的酶活性; 修饰CNTs可增大酶吸附量和酶电极对葡萄糖的响应电流, 但ESAi随CNTs修饰量的增大而降低; Au电极上电镀金后, 酶吸附量和酶电极对葡萄糖的响应电流亦增大, 但ESAi与裸金电极上的基本一致.

关键词: 电化学石英晶体阻抗, 葡萄糖氧化酶吸附, 酶比活性(ESA), 碳纳米管, 电镀金

Abstract: The electrochemical quartz crystal microbalance (EQCM) technique was employed to monitor in situ the adsorption of glucose oxidase (GOD) onto bare Au, Au-electroplated, and carbon nanotubes(CNTs)-modified Au electrodes. The enzymatic specific activities (ESAi) for GOD adsorbed on various surfaces were measured from the mass of adsorbed GOD obtained via EQCM characterization and the charge for oxidation of enzymatically generated H2O2. The results showed that GOD could adsorb on the investigated surfaces with different adsorption amounts, and the adsorbed GOD was enzymatically active to some degree; the modification of CNTs increased the adsorption amount of GOD and the response current to glucose, but the ESAi decreased with the increase of the amount of modified CNTs; the Au electroplating on Au electrode also increased the response current to glucose, but the ESAi changed little after the electroplating.

Key words: Electrochemical quartz crystal impedance, GODadsorption, Enzymatic specific activity(ESA), Carbon nanotubes, Au electroplating