物理化学学报 >> 2008, Vol. 24 >> Issue (01): 25-31.doi: 10.3866/PKU.WHXB20080105

研究论文 上一篇    下一篇

羟丙哌嗪分子印迹的固相萃取

耿利娜; 康宁; 宁周云; 武利庆; 李琰; 孙立权; 罗爱芹   

  1. 北京理工大学生命科学与技术学院, 北京 100029; 中国计量科学研究院, 北京 100013
  • 收稿日期:2007-10-15 修回日期:2007-11-13 发布日期:2008-01-05
  • 通讯作者: 罗爱芹 E-mail:bitluo@bit.edu.cn

Solid Phase Extraction of Dropropizine Using Molecularly Imprinted Polymers

GENG Li-Na; KANG Ning; NING Zhou-Yun; WU Li-Qing; LI Yan; SUN Li-Quan; LUO Ai-Qin   

  1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, P. R. China; National Institute of Metrology, Beijing 100013, P. R. China
  • Received:2007-10-15 Revised:2007-11-13 Published:2008-01-05
  • Contact: LUO Ai-Qin E-mail:bitluo@bit.edu.cn

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摘要： 以L-羟丙哌嗪为模板分子, α-甲基丙烯酸为功能单体, 三羟甲基丙烷三甲基丙烯酸酯为交联剂, 合成了羟丙哌嗪分子印迹聚合物, 利用该聚合物进行羟丙哌嗪固相萃取应用研究. 实验证明, 所合成的分子印迹聚合物具有良好的识别萃取模板分子羟丙哌嗪的能力, 能够用于羟丙哌嗪的富集浓缩, 而空白聚合物却不具备这样的特性. 考察了模板分子与其结构类似物苄基哌嗪、N-异丙基哌嗪和乙氧基羰基哌嗪的固相萃取分离情况, 研究表明, 虽然其中的苄基哌嗪在印迹材料上具有最强的保留, 但是通过先后使用不同的洗脱溶液实现了模板分子与苄基哌嗪的选择性分离.探讨了印迹识别机理,并用PM3 半经验计算机模拟方法进行了辅助分析验证.

关键词: 羟丙哌嗪分子印迹, 固相萃取分离, 结构类似物, PM3 计算机模拟方法

Abstract: Molecularly imprinted polymer (MIP) against dropropizine was synthesized using dropropizine as template, α-methyl acrylic acid as functional monomer, and trimethylolpropane trimethacrylate as crosslinking agent. Its application for separation and enrichment in solid phase extraction was investigated. It was found that, the imprinted polymer exhibited satisfactory performance in recognition and enrichment towards template, but it was not the case with non-imprinted polymer. The retention and separation of imprinted molecule with its structural analogues were also studied. The selectively separation was proved to be successful. The mechanism of imprinted recognition was also discussed here. With the help of PM3 semi-empirical method, the possible MIP system was simulated and compared with experimental results.

Key words: Dropropizine molecularly imprinted polymer, Solid phase extraction separation, Structural analogue, PM3 computer simulation method