Abstract: The multilayer films of horseradish peroxidase(HRP) were fabricated by layer-by-layer assembly of HRP and poly(sodium-p-styrenesulfonate)(PSS) on PET(polyethylene glycol terephthalate) and gold electrode surface. The activity of the assembly films was investigated. The surface topography was characterized by atomic force microscope (AFM). The relationship between the activity of the immobilized HRP with the surface roughness and topography was also studies. Cyclic voltammetry (CV) was used for the study of electrochemical properties of the films. The enzyme electrode of mltilayer films was sensitive for the reduction of hydrogen peroxide in the methylene blue (MB) solution. The response current is linear with the concentration of H2O2 in the concentration range of 0.2～5.0 mmol•L－1.

Key words: Horseradish peroxidase(HRP), Self-assembled monolayer, Atomic force microscope(AFM), Enzyme electrode, H₂O₂