物理化学学报 >> 1999, Vol. 15 >> Issue (12): 1064-1069.doi: 10.3866/PKU.WHXB19991203

研究论文 上一篇    下一篇

BSA-羟基磷灰石可溶性复合物的FTIR光谱

沈玉华, 杨展澜, 吴瑾光   

  1. 北京大学化学与分子工程学院，北京大学-香港大学稀土材料和生物无机化学联合实验室，北京 100871
  • 收稿日期:1999-01-29 修回日期:1999-04-27 发布日期:1999-12-15
  • 通讯作者: 吴瑾光

FT-IR for the Soluble Complexes of Bovine Serum Albumin Reacted with Calcium Hydroxyapatites

Shen Yu-Hua, Yang Zhan-Lan, Wu Jin-Guang   

  1. College of Chemistry and Molecular Engineering,Peking University-The University of Hong Kong Joint Laboratory in rare Earth Materials and Bioinorganic Chemistry,Peking University,Beijing 100871
  • Received:1999-01-29 Revised:1999-04-27 Published:1999-12-15
  • Contact: Wu Jin-Guang

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摘要：

利用富立叶变换红外光谱对水溶性牛血清白蛋白(BSA)-羟基磷灰石复合物的组成及结构进行了研究. 结果表明, 其组成具有非化学计量的性质, 且复合物中BSA与羟基磷灰石间存在相互作用. 从而增加了羟基磷灰石在水中的溶解度. 正是由于羟基磷灰石与蛋白质形成了水溶性的复合物,使羟基磷灰石在蛋白质结构的基质上成核和自组装成为可能,从而引起和促进了生物矿化过程.

关键词: 牛血清白蛋白, 羟基磷灰石, Ft-IR光谱, 相互作用, 生物矿化

Abstract:

Calcium hydroxyapatite [Ca 10 (OH) 2(PO 4) 6]and protein are main compositions of bones and tooth of animals. The interaction of protein with calcium hydroxyapatite plays an important role in biomineralization process. In this paper, a series of soluble bovine serum albumin (BSA)-calcium hydroxyapatite complexes in aqueous solution produced by different initial mole ratio of solid calcium hydroxyapatite and BSA were studied using FTIR spectroscopy. The results show that the composition of the soluble BSA-calcium hydroxyapatite complexes is nonstoichiometric. Calcium hydroxyapatite may be involved in hydrogen bonding to and coordinating with BSA in the soluble complexes. The interaction of BSA with calcium hydroxyapatite increased the solubility of calcium hydroxyapatite in aqueous solution, which is helpful for the formation of self organized structure of protein calcium hydroxyapatite complexes.

Key words: Bovine serum albumin, Calcium hydroxyapatite, FT-IR spectroscopy, Interaction, Biomineralization